|
|
| Acesso ao texto completo restrito à biblioteca da Biblioteca Rui Tendinha. Para informações adicionais entre em contato com biblioteca@incaper.es.gov.br. |
Registro Completo |
Biblioteca(s): |
Biblioteca Rui Tendinha. |
Data corrente: |
13/01/2015 |
Data da última atualização: |
13/01/2015 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
MARRACCINI, P.; VINECKY, F.; ALVES, G. S. C.; RAMOS, H. J. O.; ELBELT, S.; VIEIRA, N. G.; CARNEIRO, F. A.; SUJII, P. S.; ALEKCEVETCH, J. C.; SILVA, V. A.; DaMATTA, F. M.; FERRÃO, M. A. G.; LEROY, T.; POT, D.; VIEIRA, L. G. E.; SILVA, F. R. da; ANDRADE, A. C. |
Afiliação: |
Maria Amélia Gava Ferrão, Incaper/Embrapa Café. |
Título: |
Differentially expressed genes and proteins upon drought acclimation in tolerant and sensitive genotypes of Coffea canephora. |
Ano de publicação: |
2012 |
Fonte/Imprenta: |
Journal of Experimental Botany, Oxford v. 63, n. 11, p. 4191-4212, 2012. |
Idioma: |
Inglês |
Conteúdo: |
The aim of this study was to investigate the molecular mechanisms underlying drought acclimation in coffee plants by the identification of candidate genes (CGs) using different approaches. The first approach used the data generated during the Brazilian Coffee expressed sequence tag (EST) project to select 13 CGs by an in silico analysis (electronic northern). The second approach was based on screening macroarrays spotted with plasmid DNA (coffee ESTs) with separate hybridizations using leaf cDNA probes from drought-tolerant and susceptible clones of Coffea canephora var. Conilon, grown under different water regimes. This allowed the isolation of seven additional CGs. The third approach used two-dimensional gel electrophoresis to identify proteins displaying differential accumulation in leaves of drought-tolerant and susceptible clones of C. canephora. Six of them were characterized by MALDI-TOF-MS/MS (matrix-assisted laser desorption-time of flight-tandem mass spectrometry) and the corresponding proteins were identified. Finally, additional CGs were selected from the literature, and quantitative real-time polymerase chain reaction (qPCR) was performed to analyse the expression of all identified CGs. Altogether, >40 genes presenting differential gene expression during drought acclimation were identified, some of them showing different expression profiles between drought-tolerant and susceptible clones. Based on the obtained results, it can be concluded that factors involved a complex network of responses probably involving the abscisic signalling pathway and nitric oxide are major molecular determinants that might explain the better efficiency in controlling stomata closure and transpiration displayed by drought-tolerant clones of C. canephora. MenosThe aim of this study was to investigate the molecular mechanisms underlying drought acclimation in coffee plants by the identification of candidate genes (CGs) using different approaches. The first approach used the data generated during the Brazilian Coffee expressed sequence tag (EST) project to select 13 CGs by an in silico analysis (electronic northern). The second approach was based on screening macroarrays spotted with plasmid DNA (coffee ESTs) with separate hybridizations using leaf cDNA probes from drought-tolerant and susceptible clones of Coffea canephora var. Conilon, grown under different water regimes. This allowed the isolation of seven additional CGs. The third approach used two-dimensional gel electrophoresis to identify proteins displaying differential accumulation in leaves of drought-tolerant and susceptible clones of C. canephora. Six of them were characterized by MALDI-TOF-MS/MS (matrix-assisted laser desorption-time of flight-tandem mass spectrometry) and the corresponding proteins were identified. Finally, additional CGs were selected from the literature, and quantitative real-time polymerase chain reaction (qPCR) was performed to analyse the expression of all identified CGs. Altogether, >40 genes presenting differential gene expression during drought acclimation were identified, some of them showing different expression profiles between drought-tolerant and susceptible clones. Based on the obtained results, it can be concluded that factors involved a... Mostrar Tudo |
Palavras-Chave: |
Candidate gene; Coffea canephora; Differential expression; Drought acclimation; Genética; Proteomic; Proteômica; Real time PCR. |
Thesagro: |
Café; Coffea canephora; Genetics. |
Categoria do assunto: |
-- |
Marc: |
LEADER 03034naa a2200445 a 4500 001 1004907 005 2015-01-13 008 2012 bl uuuu u00u1 u #d 100 1 $aMARRACCINI, P. 245 $aDifferentially expressed genes and proteins upon drought acclimation in tolerant and sensitive genotypes of Coffea canephora.$h[electronic resource] 260 $c2012 520 $aThe aim of this study was to investigate the molecular mechanisms underlying drought acclimation in coffee plants by the identification of candidate genes (CGs) using different approaches. The first approach used the data generated during the Brazilian Coffee expressed sequence tag (EST) project to select 13 CGs by an in silico analysis (electronic northern). The second approach was based on screening macroarrays spotted with plasmid DNA (coffee ESTs) with separate hybridizations using leaf cDNA probes from drought-tolerant and susceptible clones of Coffea canephora var. Conilon, grown under different water regimes. This allowed the isolation of seven additional CGs. The third approach used two-dimensional gel electrophoresis to identify proteins displaying differential accumulation in leaves of drought-tolerant and susceptible clones of C. canephora. Six of them were characterized by MALDI-TOF-MS/MS (matrix-assisted laser desorption-time of flight-tandem mass spectrometry) and the corresponding proteins were identified. Finally, additional CGs were selected from the literature, and quantitative real-time polymerase chain reaction (qPCR) was performed to analyse the expression of all identified CGs. Altogether, >40 genes presenting differential gene expression during drought acclimation were identified, some of them showing different expression profiles between drought-tolerant and susceptible clones. Based on the obtained results, it can be concluded that factors involved a complex network of responses probably involving the abscisic signalling pathway and nitric oxide are major molecular determinants that might explain the better efficiency in controlling stomata closure and transpiration displayed by drought-tolerant clones of C. canephora. 650 $aCafé 650 $aCoffea canephora 650 $aGenetics 653 $aCandidate gene 653 $aCoffea canephora 653 $aDifferential expression 653 $aDrought acclimation 653 $aGenética 653 $aProteomic 653 $aProteômica 653 $aReal time PCR 700 1 $aVINECKY, F. 700 1 $aALVES, G. S. C. 700 1 $aRAMOS, H. J. O. 700 1 $aELBELT, S. 700 1 $aVIEIRA, N. G. 700 1 $aCARNEIRO, F. A. 700 1 $aSUJII, P. S. 700 1 $aALEKCEVETCH, J. C. 700 1 $aSILVA, V. A. 700 1 $aDaMATTA, F. M. 700 1 $aFERRÃO, M. A. G. 700 1 $aLEROY, T. 700 1 $aPOT, D. 700 1 $aVIEIRA, L. G. E. 700 1 $aSILVA, F. R. da 700 1 $aANDRADE, A. C. 773 $tJournal of Experimental Botany, Oxford$gv. 63, n. 11, p. 4191-4212, 2012.
Download
Esconder MarcMostrar Marc Completo |
Registro original: |
Biblioteca Rui Tendinha (BRT) |
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
Fechar
|
|
Registro Completo |
Biblioteca(s): |
Biblioteca Rui Tendinha; São Gabriel da Palha. |
Data corrente: |
03/05/2013 |
Data da última atualização: |
19/07/2021 |
Tipo da produção científica: |
Documentos |
Autoria: |
ALVES, F. L.; CAIADO, L. H. L.; ESPOSTI, M. D.; CAETANO, L. C. S.; GOMES, S. A.; SILVA, D. N. da.; COSTA, A. de F. S. da.; TÓFANO, G.; VIAL, J. G; SHALDERS, P.; BREGONCI, I. dos S.; PINHEIRO, R. E.; FRUTUOSO, N. das N.; SOUZA, M. A. de; DALMONECH, J. C. M.; HASSEM, A. de P.; LIMA, G. C. de.; RODRIGUES, O. O. de A.; MENOSSI, J. L.; MARQUES, P. C.; DARDENGO, A. C.; MARTINS, C. L.; TEBALDI, C. S. L.; CELIN, E.; BORGES JUNIOR, J. T. |
Afiliação: |
Flavio de Lima Alves, Incaper; Luiz Henrique Lima Caiado, Incaper; Marlon Dutra Degli Esposti, Incaper; Luiz Carlos Santos Caetano, Incaper; Sebastião Antônio Gomes, Incaper; Dalmo Nogueira da Silva, Incaper; Adelaide de Fátima Santana da Costa, Incaper; Gilson Tófano, Incaper; José Gilberto Vial, Incaper; Paulo Shalders, Incaper; Izaias dos Santos Bregonci, Incaper; Ricardo Eugenio Pinheiro, Incaper; Norberto das Neves Frutuoso, Incaper; Maxwel Assis de Souza, Incaper; José Clerio Moratti Dalmonech, Incaper; Aristodemos de Paiva Hassem, Incaper; Geraldo Costa de Lima, Incaper; Onofre Oliveira de Almeida Rodrigues, Incaper; José Luiz Menossi, Incaper; Paulo Cezar Marques, Incaper; Antônio Cezar Dardengo, Incaper; Caio Louzada Martins, Incaper; Carlos Sisasnei Lazarine Tebaldi, Incaper; Edimar Celin, Incaper; Jarbas Teixeira Borges Junior, Incaper. |
Título: |
Polo de laranja da região Sul-Caparaó do estado do Espirito Santo. |
Ano de publicação: |
2011 |
Fonte/Imprenta: |
Vitoria, ES: Incaper, 2011. |
Descrição Física: |
15 cm. x 27 cm. |
Série: |
(Incaper. Documentos, 199) |
ISSN: |
1519-2059 |
Idioma: |
Português |
Palavras-Chave: |
Arujem; Citricultura; Frucafé; Fundecitrus; Idaf; Sebrae; Ufrgs; Unesp. |
Categoria do assunto: |
-- |
URL: |
http://biblioteca.incaper.es.gov.br/digital/bitstream/item/197/1/folder-polo-laranja.pdf
|
Marc: |
LEADER 01354nam a2200517 a 4500 001 1000033 005 2021-07-19 008 2011 bl uuuu u0uu1 u #d 022 $a1519-2059 100 1 $aALVES, F. L. 245 $aPolo de laranja da região Sul-Caparaó do estado do Espirito Santo. 260 $aVitoria, ES: Incaper$c2011 300 $c15 cm. x 27 cm. 490 $a(Incaper. Documentos, 199) 653 $aArujem 653 $aCitricultura 653 $aFrucafé 653 $aFundecitrus 653 $aIdaf 653 $aSebrae 653 $aUfrgs 653 $aUnesp 700 1 $aCAIADO, L. H. L. 700 1 $aESPOSTI, M. D. 700 1 $aCAETANO, L. C. S. 700 1 $aGOMES, S. A. 700 1 $aSILVA, D. N. da. 700 1 $aCOSTA, A. de F. S. da. 700 1 $aTÓFANO, G. 700 1 $aVIAL, J. G 700 1 $aSHALDERS, P. 700 1 $aBREGONCI, I. dos S. 700 1 $aPINHEIRO, R. E. 700 1 $aFRUTUOSO, N. das N. 700 1 $aSOUZA, M. A. de 700 1 $aDALMONECH, J. C. M. 700 1 $aHASSEM, A. de P. 700 1 $aLIMA, G. C. de. 700 1 $aRODRIGUES, O. O. de A. 700 1 $aMENOSSI, J. L. 700 1 $aMARQUES, P. C. 700 1 $aDARDENGO, A. C. 700 1 $aMARTINS, C. L. 700 1 $aTEBALDI, C. S. L. 700 1 $aCELIN, E. 700 1 $aBORGES JUNIOR, J. T.
Download
Esconder MarcMostrar Marc Completo |
Registro original: |
Biblioteca Rui Tendinha (BRT) |
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
Fechar
|
Expressão de busca inválida. Verifique!!! |
|
|